Amino Acid Type Identification Experiments





Protonated Proteins

Name Pulse Sequence Au Program Reference
Sequential Cβ Edited [1H-15N] HSQC BECE splitBECE 1
Sequential Cγ Edited [1H-15N] HSQC GACE 1
Intra Cβ Edited [1H-15N] HSQC BECEi splitBECE 1
Sequential Cβ Edited [1H-15N] HSQC (starting from HN) BECE2 splitBECE2 2
Intra Cβ Edited [1H-15N] HSQC (starting from HN) BECEi2 splitBECE2 2
Cβ Edited CACO (starting at HCA) C_BECE_CACO splitBECECACO 3
Cβ Edited CON (starting at HCA) C_BECE_CON splitBECECON 3


Deuterated Proteins

Name Pulse Sequence Au Program Reference
Sequential Cβ Edited [1H-15N] HSQC (starting from HN) BECE2H splitBECE2H 2
Intra Cβ Edited [1H-15N] HSQC (starting from HN) BECEi2H splitBECE2H 2
Cβ Edited CACO (starting at CB) C_BECE_CACO splitBECECACO 3
Cβ Edited CON (starting at CB) C_BECE_CON splitBECECON 3




1.- Amino acid type identification in NMR spectra of proteins via β- and γ-carbon edited experiments. D. Pantoja-Uceda and J. Santoro J. Magn. Reson. 195, 187-195 (2008).
2.- New amino acid residue type identification experiments valid for protonated and deuterated proteins. D. Pantoja-Uceda and J. Santoro J. Biomol. NMR DOI: 10.1007/s10858-012-9665-y (2012).
3.- A suite of amino acid residue type classification pulse sequences for 13C detected NMR of proteins J. Magn. Reson. DOI: 10.1016/j.jmr.2013.06.023 (2013).