Simultaneous Measurement of N-H and Cα-Hα Coupling Constants in Proteins

D. Pantoja-Uceda and J. Santoro
Magnetic Resonance in Chemistry 48, 20-24 (2010).
  • DESCRIPTION:
    - Pulse sequence for the simultaneous measurement of N-H and Cα-Hα couplings in double labeled proteins from 2D spectra. The proposed sequence, a modification of the HN(CO)CA experiment, combines the J-modulation method and the IPAP scheme.

  • FILES (Bruker):
    - Pulse Sequence MULTIJ
    - Au Program splitMULTIJ


RESULTS

Ubiquitin (76 aa) 1.7mM 25C, AV800+CRP